Table 1.
Description of CrLEAs identified from Canavalia rosea genome.
| Name | Gene Locus | Length (aa) | Mw (kD) | PI | Instability Index (II) |
Aliphatic Index (AI) |
GRAVY | Content of Disordered aa (%) |
Subcellular Localization * |
|---|---|---|---|---|---|---|---|---|---|
| CrLEA1-1 | 03T007960 | 174 | 17.92 | 9.13 | 11.32 | 35.00 | −1.060 | 83.91 | mito: 10, nucl: 4 |
| CrLEA1-2 | 04T011772 | 192 | 20.00 | 5.38 | 45.87 | 49.53 | −0.725 | 83.33 | nucl: 7, cyto: 3 |
| CrLEA1-3 | 05T014731 | 136 | 14.98 | 9.03 | 54.57 | 60.44 | −0.992 | 94.12 | nucl: 9, mito: 3 |
| CrLEA2-1 | 01T000091 | 294 | 32.86 | 10.00 | 57.75 | 79.18 | −0.266 | 25.51 | chlo: 3, cyto: 3, E.R.: 3 |
| CrLEA2-2 | 01T000357 | 210 | 22.61 | 9.47 | 39.79 | 88.24 | 0.250 | 10.48 | chlo: 5, mito: 5 |
| CrLEA2-3 | 01T000402 | 221 | 24.59 | 9.21 | 37.80 | 103.57 | 0.078 | 18.55 | chlo: 5, cyto: 5 |
| CrLEA2-4 | 01T001888 | 207 | 22.93 | 9.32 | 34.25 | 95.12 | 0.038 | 12.08 | plas: 8, cyto: 4 |
| CrLEA2-5 | 01T003219 | 230 | 25.26 | 9.21 | 34.53 | 111.39 | 0.283 | 16.96 | plas: 4.5, vacu: 4, nucl_plas: 3 |
| CrLEA2-6 | 02T004727 | 259 | 28.57 | 9.88 | 35.46 | 82.39 | −0.232 | 18.53 | chlo: 10, mito: 3 |
| CrLEA2-7 | 02T004857 | 262 | 29.07 | 9.80 | 50.89 | 84.35 | −0.331 | 22.14 | cyto: 7 |
| CrLEA2-8 | 02T005159 | 207 | 23.10 | 9.78 | 33.03 | 100.24 | −0.048 | 13.04 | chlo: 4, plas: 4 |
| CrLEA2-9 | 02T006010 | 192 | 20.74 | 9.28 | 13.81 | 106.98 | 0.272 | 6.25 | vacu: 4, E.R.: 3 |
| CrLEA2-10 | 02T006879 | 190 | 21.16 | 6.83 | 28.11 | 96.42 | −0.112 | 6.32 | nucl: 8, chlo: 3 |
| CrLEA2-11 | 02T006881 | 248 | 27.67 | 10.05 | 33.42 | 94.68 | −0.042 | 14.52 | cyto: 8 |
| CrLEA2-12 | 02T007016 | 233 | 26.24 | 9.41 | 48.70 | 97.38 | −0.033 | 21.46 | cyto: 8 |
| CrLEA2-13 | 02T007186 | 250 | 27.17 | 9.34 | 36.97 | 90.88 | −0.004 | 16.4 | chlo: 8, mito: 4 |
| CrLEA2-14 | 03T008517 | 227 | 25.78 | 9.40 | 36.41 | 89.30 | −0.131 | 7.49 | golg: 4.5, golg_plas: 4.5, plas: 3.5, E.R.: 3 |
| CrLEA2-15 | 03T008518 | 228 | 26.55 | 9.48 | 37.63 | 81.18 | −0.291 | 10.09 | chlo: 10, pero: 3 |
| CrLEA2-16 | 03T008519 | 209 | 23.77 | 9.71 | 36.98 | 97.32 | 0.018 | 7.18 | cyto: 11 |
| CrLEA2-17 | 03T009222 | 197 | 21.48 | 9.76 | 31.71 | 110.71 | 0.356 | 7.61 | chlo: 6, E.R.: 4 |
| CrLEA2-18 | 03T009270 | 235 | 26.49 | 8.98 | 33.86 | 103.19 | 0.329 | 6.81 | cyto: 10 |
| CrLEA2-19 | 03T010271 | 222 | 24.12 | 9.64 | 24.57 | 121.08 | 0.232 | 15.77 | nucl: 6 |
| CrLEA2-20 | 03T010272 | 195 | 21.68 | 6.95 | 23.09 | 100.41 | 0.074 | 6.67 | E.R.: 4, mito: 3, extr: 3 |
| CrLEA2-21 | 03T010452 | 176 | 19.07 | 5.86 | 26.80 | 113.47 | 0.181 | 6.25 | plas: 6, vacu: 5 |
| CrLEA2-22 | 04T011627 | 200 | 23.20 | 9.40 | 35.70 | 104.65 | 0.115 | 0.5 | extr: 3, vacu: 3, E.R.: 3 |
| CrLEA2-23 | 04T011636 | 228 | 26.46 | 9.57 | 34.97 | 81.67 | −0.233 | 11.84 | chlo: 8 |
| CrLEA2-24 | 04T011638 | 210 | 23.82 | 9.63 | 47.73 | 101.52 | 0.099 | 6.19 | cyto: 7 |
| CrLEA2-25 | 04T012994 | 249 | 27.84 | 9.90 | 38.97 | 101.37 | −0.087 | 20.48 | cyto: 7, nucl: 3, E.R.: 3 |
| CrLEA2-26 | 04T013772 | 225 | 25.61 | 9.42 | 41.04 | 76.62 | −0.399 | 11.11 | chlo: 14 |
| CrLEA2-27 | 05T014592 | 322 | 35.41 | 10.11 | 58.09 | 71.71 | −0.333 | 31.68 | chlo: 3, vacu: 3 |
| CrLEA2-28 | 05T016584 | 272 | 29.42 | 9.60 | 42.56 | 84.19 | −0.214 | 32.35 | nucl: 5, chlo: 4, plas: 3 |
| CrLEA2-29 | 05T016770 | 202 | 23.15 | 9.75 | 44.59 | 107.13 | 0.049 | 11.39 | chlo: 5, mito: 3 |
| CrLEA2-30 | 06T017171 | 233 | 26.69 | 10.13 | 39.27 | 102.45 | −0.009 | 19.31 | cyto: 5, plas: 4 |
| CrLEA2-31 | 06T017436 | 320 | 35.70 | 4.80 | 25.33 | 91.97 | −0.437 | 16.25 | cyto: 9 |
| CrLEA2-32 | 06T017480 | 273 | 30.26 | 9.43 | 50.16 | 89.56 | −0.200 | 19.41 | cyto: 4, chlo: 3, plas: 3 |
| CrLEA2-33 | 06T018775 | 257 | 28.95 | 10.17 | 49.92 | 99.69 | −0.162 | 23.35 | nucl: 6, cyto: 5 |
| CrLEA2-34 | 07T020998 | 211 | 24.12 | 9.10 | 30.10 | 95.59 | 0.045 | 6.64 | extr: 5, chlo: 3, E.R.: 3 |
| CrLEA2-35 | 07T020999 | 499 | 57.65 | 9.51 | 31.38 | 89.80 | −0.086 | 2.2 | plas: 7.5, golg_plas: 6, golg: 3.5 |
| CrLEA2-36 | 07T021162 | 247 | 27.83 | 9.15 | 54.28 | 97.41 | −0.057 | 13.77 | plas: 4, golg: 3 |
| CrLEA2-37 | 07T021185 | 308 | 34.10 | 9.58 | 62.03 | 62.27 | −0.514 | 25.32 | chlo: 3, plas: 3, E.R.: 3 |
| CrLEA2-38 | 08T022453 | 223 | 24.65 | 9.50 | 27.00 | 97.89 | 0.155 | 15.25 | chlo: 5, cyto: 3 |
| CrLEA2-39 | 08T022523 | 219 | 24.64 | 9.74 | 53.37 | 106.80 | 0.185 | 5.94 | E.R.: 5 |
| CrLEA2-40 | 08T022549 | 252 | 27.66 | 6.83 | 38.09 | 110.32 | 0.311 | 5.56 | cyto: 7 |
| CrLEA2-41 | 08T023188 | 317 | 35.77 | 9.67 | 57.68 | 82.93 | −0.391 | 36.59 | chlo: 4, nucl: 3, mito: 3 |
| CrLEA2-42 | 09T024657 | 244 | 27.90 | 7.50 | 40.73 | 100.20 | 0.075 | 18.03 | chlo: 6 |
| CrLEA2-43 | 09T024732 | 184 | 19.82 | 9.68 | 21.51 | 121.25 | 0.497 | 4.89 | vacu: 5, extr: 3 |
| CrLEA2-44 | 09T024733 | 186 | 20.56 | 8.99 | 14.50 | 107.37 | 0.354 | 3.76 | extr: 4, vacu: 4 |
| CrLEA2-45 | 09T024798 | 220 | 24.57 | 8.97 | 38.64 | 104.95 | 0.119 | 6.82 | chlo: 4 |
| CrLEA2-46 | 09T025174 | 255 | 27.99 | 10.30 | 36.23 | 87.22 | −0.138 | 18.04 | chlo: 8, mito: 5 |
| CrLEA2-47 | 09T025255 | 308 | 34.13 | 9.93 | 58.73 | 72.37 | −0.347 | 29.55 | chlo: 4, E.R.: 3 |
| CrLEA2-48 | 09T025271 | 228 | 25.83 | 9.63 | 28.59 | 105.39 | 0.153 | 10.96 | vacu: 3 |
| CrLEA2-49 | 10T025889 | 190 | 21.52 | 9.52 | 31.74 | 88.79 | −0.412 | 9.47 | vacu: 5, extr: 3, E.R.: 3 |
| CrLEA2-50 | 10T025950 | 185 | 20.54 | 8.80 | 27.57 | 114.27 | 0.268 | 4.86 | extr: 3, vacu: 3, E.R.: 3 |
| CrLEA2-51 | 10T026128 | 183 | 20.17 | 9.67 | 34.72 | 89.40 | 0.371 | 5.46 | chlo: 7, vacu: 3 |
| CrLEA2-52 | 10T026178 | 192 | 21.44 | 8.41 | 50.06 | 97.40 | 0.068 | 18.23 | cyto: 7.5, cyto_nucl: 6, nucl: 3.5 |
| CrLEA2-53 | 10T026179 | 204 | 22.68 | 10.14 | 39.68 | 94.61 | −0.012 | 10.29 | chlo: 6, E.R.: 4 |
| CrLEA2-54 | 10T026917 | 221 | 24.71 | 9.14 | 41.43 | 97.47 | −0.089 | 18.55 | cyto: 7, cysk: 4 |
| CrLEA2-55 | 11T027970 | 382 | 42.52 | 4.97 | 23.26 | 96.83 | −0.283 | 17.02 | cyto: 8, nucl: 3 |
| CrLEA2-56 | 11T028314 | 258 | 29.48 | 10.11 | 52.91 | 92.91 | −0.151 | 20.93 | chlo: 11 |
| CrLEA2-57 | 11T028577 | 244 | 27.78 | 10.10 | 47.89 | 104.18 | −0.164 | 18.85 | cyto: 5, nucl: 3 |
| CrLEA2-58 | 11T029314 | 264 | 29.23 | 9.27 | 46.36 | 88.98 | −0.062 | 18.56 | cyto: 8, chlo: 4 |
| CrLEA2-59 | 11T029316 | 264 | 29.19 | 9.11 | 47.73 | 90.45 | −0.031 | 18.18 | cyto: 8, chlo: 3 |
| CrLEA2-60 | 11T029318 | 264 | 29.43 | 9.23 | 48.32 | 83.45 | −0.146 | 18.18 | cyto: 7.5, cyto_nucl: 4.5, chlo: 4 |
| CrLEA3-1 | 01T003524 | 106 | 11.73 | 10.09 | 56.38 | 89.25 | −0.325 | 46.23 | chlo: 12 |
| CrLEA3-2 | 02T007058 | 84 | 9.69 | 9.34 | 40.26 | 68.57 | −0.664 | 39.29 | chlo: 5, mito: 4, nucl: 3 |
| CrLEA3-3 | 03T008662 | 97 | 10.57 | 9.91 | 43.60 | 76.39 | −0.300 | 47.42 | cyto: 7, chlo: 6 |
| CrLEA3-4 | 04T012522 | 97 | 10.20 | 9.70 | 27.95 | 77.63 | −0.252 | 46.39 | chlo: 13 |
| CrLEA3-5 | 04T013832 | 101 | 11.26 | 9.25 | 44.98 | 66.63 | −0.655 | 48.51 | mito: 8, chlo: 3 |
| CrLEA3-6 | 05T015908 | 105 | 11.32 | 9.88 | 60.57 | 69.62 | −0.456 | 54.29 | cyto: 8, mito: 5 |
| CrLEA4-1 | 03T008401 | 310 | 34.21 | 7.05 | 28.99 | 49.00 | −1.093 | 65.16 | chlo: 6, nucl: 6 |
| CrLEA4-2 | 04T011481 | 427 | 46.51 | 6.04 | 36.13 | 43.89 | −1.296 | 77.52 | nucl: 12 |
| CrLEA5-1 | 02T006409 | 95 | 10.26 | 5.87 | 48.75 | 41.16 | −1.355 | 87.37 | nucl: 6, cyto: 6 |
| CrLEA5-2 | 03T010121 | 158 | 17.54 | 9.44 | 49.86 | 48.73 | −1.237 | 72.78 | chlo: 9, nucl: 5 |
| CrLEA6-1 | 02T003983 | 82 | 9.20 | 9.20 | 47.60 | 54.76 | −1.194 | 73.17 | nucl: 6, mito: 5 |
| CrLEA6-2 | 05T014654 | 457 | 50.38 | 9.10 | 56.56 | 52.32 | −0.962 | 71.12 | nucl: 13 |
| CrDHN1 | 01T000101 | 80 | 8.58 | 6.49 | 24.98 | 51.12 | −1.068 | 48.75 | nucl: 5 |
| CrDHN2 | 02T003923 | 215 | 24.36 | 5.25 | 57.49 | 51.63 | −1.520 | 79.07 | nucl: 12 |
| CrDHN3 | 02T003929 | 190 | 19.95 | 8.81 | 30.05 | 39.11 | −1.043 | 82.63 | nucl: 13 |
| CrDHN4 | 06T019348 | 220 | 25.01 | 5.45 | 43.12 | 47.41 | −1.636 | 80.91 | nucl: 11 |
| CrDHN5 | 07T021123 | 194 | 19.47 | 6.28 | −5.30 | 16.65 | −1.215 | 72.16 | nucl: 9 |
| CrSMP1 | 04T011095 | 203 | 20.80 | 4.97 | 28.89 | 82.81 | −0.185 | 17.24 | cyto: 7, chlo: 6 |
| CrSMP2 | 04T012849 | 278 | 28.83 | 5.20 | 34.95 | 78.35 | −0.357 | 25.9 | cyto: 6, chlo: 4 |
| CrSMP3 | 04T013711 | 258 | 26.15 | 4.77 | 36.17 | 74.65 | −0.286 | 17.05 | cyto: 5, chlo: 4 |
| CrSMP4 | 09T023499 | 284 | 29.44 | 4.90 | 28.98 | 79.82 | −0.137 | 25 | chlo: 8 |
| CrASR1 | 04T013503 | 235 | 25.92 | 5.79 | 40.95 | 28.00 | −1.449 | 90.64 | nucl: 6, cyto: 3 |
| CrASR2 | 07T020512 | 130 | 14.98 | 6.34 | 42.06 | 47.46 | −1.321 | 80.77 | mito: 5, nucl: 4, cyto: 3 |
| CrASR3 | 07T020519 | 114 | 13.09 | 6.41 | 36.67 | 51.58 | −1.341 | 79.82 | mito: 5, nucl: 4, cyto: 3 |
The physicochemical parameters, including molecular weight (kDa) and pI, of each CrLEA proteins were calculated using the compute pI/Mw tool of ExPASy (http://www.expasy.org/tools/, accessed on 1 March 2021). GRAVY (grand average of hydropathy) values were calculated using the PROTPARAM tool (http://web.expasy.org/protparam/, accessed on 1 March 2021). The contents of disordered amino acids (%) in each CrLEA/ASR were calculated according to the online program PrDOS (Protein DisOrder prediction System, http://prdos.hgc.jp/cgi-bin/top.cgi, accessed on 1 March 2021), with the false positive rate (FP) = 5%, and also the correspondingly disorder profile plots for each protein were shown in Figure S2. Subcellular location prediction was conducted using the WoLF_PSORT (https://www.genscript.com/wolf-psort.html, accessed on 1 March 2021). nucl: Nucleus; plas: Plasma membrane; cyto: Cytoplasm; mito: Mitochondria; ER: Endoplasmic reticulum; pero: Peroxisomal; chlo: Chloroplast; golg: Golgi; vacu: Tonoplast membrane; cysk: Cytoskeleton; extr: Extracellular region; secr: Secretory. * The scores predicted by WoLF PSORT below 3 were ignored. The scores represent the probabilities of the subcellular localization.