Figure 3.

Effects of orexin A on the expression of BMP-receptor signaling molecules in AtT20 cells. (A,B) AtT20 cells treated with orexin (ORX) for 24 h were extracted, and the mRNA expression levels of Alk2, Alk3, Alk6, BmprII, ActrIIa (A), Smad6, and Smad7 (B) were quantified by qPCR. The expression levels of target genes were standardized by the RPL19 level in each sample. (C) Cells were treated with ORX in serum−free media for 24 h. The cell lysates were subjected to immunoblot (IB) analysis using antibodies that detect Smad6, Smad7, and actin. Results are shown as means ±SEM of data from at least three independent experiments. Statistical analysis was performed by the unpaired t-test. * p < 0.05 vs. between the control groups.