Figure 1.

RT-qPCR of Angiotensin Convertase Enzyme type 2 in macrophages of HHT1, HHT2 and Control donors. Mononuclear cells from healthy and HHT donors were cultivated in DMEM with 10% FCS, and treated by LPS 10 ng/mL for 48 h. Cells were lysed, and RNA was extracted as described in Materials and Methods. RT-qPCR was performed for ACE2 receptor in 4 HHT2, 3 HHT1 and 4 control donor samples. Figure represents the number of cycles corresponding to the Ct of the amplification curve at similar Actin RNA amount taken as housekeeping gene. Ns—not significant; ** p < 0.01.