Skip to main content
. 2021 May 11;13:3789–3801. doi: 10.2147/CMAR.S290629

Figure 5.

Figure 5

KLF12 was a direct target of miR-138-5p. (A) The binding sites between miR-138-5 and KLF12, as well as the mutant. (B and C) Dual-luciferase reporter assay for the luciferase intensity of KLF12-wt and KLF12-mut in HGC-27 and MKN45 cells transfected with miR-NC or miR-138-5p. (D) QRT-PCR assay for the relative expression of miR-138-5p in HGC-27 and MKN45 cells transfected with miR-NC, miR-138-5p, anti-miR-NC or anti-miR-138-5p. (E and F) QRT-PCR and Western blot assays for the mRNA and protein levels of KLF12 in HGC-27 and MKN45 cells transfected with miR-NC, miR-138-5p, anti-miR-NC or anti-miR-138-5p. (G) QRT-PCR for the mRNA level of KLF12 in GC tissues (n=50) and matched adjacent normal tissues. (H) Western blot assay for the protein level of KLF12 in GC tissues and matched adjacent normal tissues. (I) Pearson correlation analysis for the correlation between expression of KLF12 mRNA and miR-138-5p in GC tissues. (J and K) QRT-PCR and Western blot assays for the mRNA and protein levels of KLF12 in GES-1, HGC-27 and MKN45 cells. *P <0.05.