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. 2021 May 15;12(5):495. doi: 10.1038/s41419-021-03748-y

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 3 — A Illustration of direct and trans-well co-culture of MΦs and MM cells. B Western blot showed in both direct and trans-well co-culture methods, the expression of BMI1 was elevated in MM-MΦs, relative to MΦs. C Western blot showed that protein levels of BMI1 were increased in MΦs upon rmSHH treatment. D Western blot showed that the GLI1/GLI2 transcriptional activity inhibitor GANT61 suppressed expression of BMI1 in both murine BM-derived and human PBMC-derived MM-MΦs. E Western blot showed that the Smo antagonist Cyclopamine reduced expression of BMI1 in both murine BM-derived and human PBMC-derived MM-MΦs. F Western blot showed c-Myc expression was increased in both murine and human derived MM-MΦs. G Western blot showed that the c-Myc inhibitor 10058-F4 reduced expression of BMI1 in both murine and human MM-MΦs. H ChIP-qPCR showed the relative occupancy of c-Myc and IgG control to BMI1 promoter region in MΦs and MM-MΦs derived from mouse and human. I Schematic of Hedgehog-c-Myc axis regulates BMI1 expression in MM-MΦs.