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. 2021 May 15;12(5):495. doi: 10.1038/s41419-021-03748-y

Fig. 7. BMI1 regulates MM-MΦ’s pro-myeloma functions in vivo.

Fig. 7

A Schematic displayed that 5TGM1 cells were injected subcutaneously into the B-NDG mice alone or with BM-derived MΦs from wt or BMI1-KO mice. B Flow cytometry showed the proportion of MΦs in the subcutaneous tumor beds 7 days after inoculation (Left panel). Tumors with wt MΦs had the most MΦs in tumor beds (right column). Statistical significance was determined by two-tailed Student t-test between tumors with 5TGM1 alone and with wt MM-MФs, or between tumors with wt and BMI1-KO MM-MФs, *P < 0.05. C Left panel showed the growth curves of 5TGM1 xenografts, wt MΦs were the most potent in promoting tumor growth. Right panel showed monoclonal protein IgG2b concentrations in murine peripheral blood, mice bearing tumors with wt MΦs had the highest concentrations of IgG2b. Statistical significance was determined by two-tailed Student t-test between groups with wt and BMI1-KO MM-MФs, *P < 0.05. D Representative IHC staining images (left images) and quantification of positive cells (right column) of epithelial cell marker CD34. Statistical significance was determined by two-tailed Student t-test between tumors with 5TGM1 alone and with wt MM-MФs, or between tumors with wt and BMI1-KO MM-MФs, *P < 0.05.