FIGURE 3.

Inhibition of miR‐140‐3p in CRC cells promoted proliferation, migration, and invasion, and suppressed apoptosis. HCT 116 cells were transfected with miR‐140‐3p inhibitor or negative control. (A) miR‐140‐3p expression in HCT 116 cells following miR‐140‐3p inhibitor or negative control transfection. (B) Cell viability. (C) Transwell migration assay. Scale bar, 100 μm. (D) Effects of miR‐140‐3p silencing on cell invasion. Scale bar, 100 μm. (E) Vimentin expression was analyzed by immunofluorescence staining. Scale bar, 50 μm. (F) Inhibition of miR‐140‐3p affected the expression of EMT biomarkers. (G) Apoptosis of HCT 116 cells was determined by FACS. (H) Apoptosis was evaluated by Hoechst staining. Scale bar, 50 μm. (I) The levels of apoptosis‐related markers following miR‐140‐3p inhibition. Data in A, C, D, F, G, H, and I were present by mean ± SD (n = 3, two‐tailed unpaired t test), with repeated measures ANOVA for panel B