FIGURE 8.

miR‐140‐3p suppressed tumor growth and liver metastasis in LoVo cell xenograft mouse model. The nude mice were subjected to subcutaneous injection of LoVo cells: (A) Tumor volume was monitored from days 5 to day 35. (B) The images of tumor at 35 days after subcutaneous injection. (C) miR‐140‐3p, BCL9, and BCL2 expression in tumor tissues was detected by real‐time PCR. (D) TUNEL staining of tumor tissue sections to detect apoptosis. Thirty‐five days after tail vein injection of LoVo cells: (E) The liver morphology and nodules were presented. (F) HE staining of metastatic liver tissues and quantitation of (F) by counting hepatic metastatic nodules. The metastatic nodules were indicated with black arrows. Data were presented by mean ± SD (n = 6), with repeated measures ANOVA for A, and two‐tailed unpaired t test for C and F