Figure 2.

Large-scale structural rearrangements of PFL and its activating enzyme. (A) Reaction catalyzed by PFL-AE. (B) Docking studies suggest hinged motion of the radical domain in PFL (blue cartoon, PDB accession code: 2PFL) together with loop motions in PFL-AE (PDB accession code: 3CB8, mobile loop region highlighted on surface) facilitate substrate positioning. The outline of PFL is predominantly shown as a solid grey shape to help distinguish the radical domain. The catalytic glycine residue is rendered in yellow spheres for emphasis. Note the complex depicted here is not the original docking model and was generated manually for visualization of described computational results.