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. 2021 Apr 30;13(9):2174. doi: 10.3390/cancers13092174

Figure 2.

Figure 2

COT mediates IL-33-induced LPIN1 expression. (A) MCF7 cells were transfected with different amounts of Myc-COT, incubated for 48 h, harvested, and subjected to immunoblotting. (B,C) MCF7 cells were transfected with Myc-COT (B) or siRNA-COT (C). At 48 h after transfection, the cells were serum starved for 24 h, then treated with 25 ng/mL IL-33 for 24 h, harvested, and lysed. Proteins in the whole cell lysates were separated using SDS-PAGE and subjected to immunoblotting. (D,E) MCF7 cells were serum starved for 24 h, pretreated with the indicated concentrations of TKI for 2 h, exposed to 25 ng/mL IL-33 for 24 h, and harvested. The mRNA levels were assessed using RT-PCR and real-time PCR. Columns, mean of triplicate samples. bars, S.D. **, p < 0.01, when compared to the control cells. (F) MCF7 cells were serum starved for 24 h, pretreated with the indicated concentrations of TKI for 2 h, then exposed to 25 ng/mL IL-33 for 24 h, harvested, and lysed. Proteins in the whole cell lysates were separated using SDS-PAGE and subjected to immunoblotting.