Figure 5.

IL-33-induced LPIN1 promotes neoplastic cell transformation. (A) MCF7 cells were treated with different concentrations of SP600125 in the absence or presence of 25 ng/mL IL-33 for 48 h, following which the cell proliferation was estimated using the BrdU incorporation assay. (B,C) MCF7 cells were exposed to 25 ng/mL IL-33 with/without SP600125, as indicated in the soft agar matrix, and incubated at 37 °C in a 5% CO₂-humidified atmosphere for 14 days. (D) MCF7 cells were treated with different concentrations of propranolol in the absence or presence of 25 ng/mL IL-33 for 48 h, following which the cell proliferation was estimated using a BrdU incorporation assay. (E,F) MCF7 cells were exposed to 25 ng/mL IL-33, with/without propranolol, as indicated in the soft agar matrix, and incubated at 37 °C in a 5% CO₂-humidified atmosphere for 14 days. (A,C,D,F) Columns, mean of triplicate samples. bars, S.D. *, p < 0.05, **, p < 0.01, and ***, p < 0.001.