Table 4.
Oligonucleotides used in this study.
| No | Designation | Sequence |
|---|---|---|
| 1 | CFPSmSaP | gccccggGGTCGACTTACTTGTACAGCTCG |
| 2 | CkorCD | cgacatgtTTATGTTCGGTCATGGTTTC |
| 3 | CkorCG | gcgcatgcCTTAAAGGAGGTGCATAGGT |
| 4 | FLAGVirDR | ccaagcttTTATGCCGCTTCAGCCAAGC |
| 5 | kasmob1 | cggaattcacatgtTTCTCGTTGGAGGGTGATCA |
| 6 | KFRCBSD | tcgacaagcttCCGCT |
| 7 | KFRCBSG | CTAGAGCGGaagcttg |
| 8 | kfrCFL | gcaagctttggaattCATGACCGAACATAAGGCCGA |
| 9 | kfrCIR | cggtcgacttacccgggAGCTCCGCTTTTGCCCATTC |
| 10 | kfrCIIF | cggaattcATGGGCAAAAGCGGAGCTGA |
| 11 | kfrCIIR | cggtcgacTTAcccgggCCGCTCTAGATCGTCTTCAT |
| 12 | kfrCmutF | TGTCGcgGccgCGCTGGCGATGGGCG |
| 13 | kfrCmutR | CCAGCGcggCcgCGACAATCAGATAAGGCTGGTCA |
| 14 | kfrCrbsF | cggaattcagatctaaggagGAAACCATGACCGAACATAA |
| 15 | KfrCRK2N | gcgaattcaTGAGCAGCTACAGCAGAG |
| 16 | KfrCRK2R | gcggtaccTTAGCTGGGCTTGTTTGAC |
| 17 | KfrCRKst | cgcccgggGCTGGGCTTGTTTGACAGG |
| 18 | KfrCstop | cggtcgacCCGCTCTAGATCGTCTTCAT |
| 19 | KfrCXbaF | cgTCTAGAGCGGAAGCTTGCGG |
| 20 | LinkSceF | tagggataacagggtaatgtac |
| 21 | LinkSceR | attaccctgttatccctagtac |
| 22 | mobC1 | cggaattcATGGCAAAGAGCTATCGGATCG |
| 23 | mobC2 | cggtcGACTCGCTTAACTCGGCCTTTCA |
| 24 | mobCT | gcgagctccTTCATCGATCCCCCACTTG |
| 25 | nic1 | cggaattcATGAATAAGGGCTATGACACTCTAGCCGGG |
| 26 | nic2 | cggtcgacTTATCTCTCGTCTTCGTCCC |
| 27 | Nic2k | gcgagctcgTCTCTCGTCTTCGTCCCTCTCTGATTTTGC |
| 28 | OKFRCD2 | tcgacggtaccagcggcttcaCCGCT |
| 29 | OKFRCG2 | CTAGAGCGGtgaagccgctggtaccg |
| 30 | OPETD | GATCGTGCAGC |
| 31 | OPETG | CATGGCTGCAC |
| 32 | pGBT30R | CTCTTCCGCATAAACGCTTC |
| 33 | podst4F | aattggggctcc |
| 34 | podst4R | agctggagctcc |
| 35 | T7TERR | gcgtcgacCAAAAAACCCCTCAAGACCC |
| 36 | TerpKKKF | cgcggtaccctcgagcccgggATCAGAACGCAGAAGCGGTC |
| 37 | TerpKKR | cgcggtaccagtactGGCTTGTAGATATGACGACAG |
| 38 | TraGEcoF | gcgaattcATGAAGATCAAGATGAACAAC |
| 39 | TraGKpnR | gcggtacCTCATATCGTGATGCCCTCCC |
| 40 | TraGRK2F | gcgaattcATGAAGAACCGAAACAACGCC |
| 41 | TraGRK2R | gcggtacCTCATATCGTGATCCCCTCC |
| 42 | TraGRKst | cgcccgggTATCGTGATCCCCTCCCCTTC |
| 43 | TraGSmaR | cgcccgggTATCGTGATGCCCTCCC |
| 44 | virD4Gm | gcggattcATGACCCAGAATTCAAACGGACAC |
| 45 | virD4Kpn | cgggtaCCTTATGCCGCTTCAGCCAAGCCATT |
| 46 | virD4N | cggagctcCTGCCGCTTCAGCCAAGCCATTAA |
| 47 | VirDfr2F | gcgaattcTTGCGTGAAACATATGGG |
| 48 | VirDfrBR | gcggaTCCTTATGCCGCTTCAGCCAAG |
| 49 | VirDMunF | cgcaattgATGACCCAGAATTCAAACG |
| 50 | TProLyzF | gcgaattctacgtactcgagagatctACATGTGGTACCAACCACC |
| 51 | TProLyzR | gcgtcgacCCATGGATAATAGTTAACGAG |
| 52 | delincF | CGAGGATGAGGCATATAAACAGGCTAATAAACCAAAGGGTTGAGCATATGATTCCGGGGATCCGTCGACC |
| 53 | delincR | CCGCTGAGGTCTGCCCCTTTACCACTCATTCAGCCACCCCCATTTTTTCATGTAGGCTGGAGCTGCTTCG |
| 54 | delnicF | TCGCCGGTTTGCTTCAACGCAACTTAAACAAGTGGGGGATCGATGAATAAATTCCGGGGATCCGTCGACC |
| 55 | delnicR | GAACGCTAAATACCTGAAAACAAAAACCGGCCAACAGGCCGGTTTTTTTATGTAGGCTGGAGCTGCTTCG |
| 56 | delvirF | TAACGGAGATTTACTATGACCCAGAATTCAAACGGACACAAATGGCGTAAATTCCGGGGATCCGTCGACC |
| 57 | delvirR | TATGTTTTTTCCTGTGCAATATTTGCCATTTCAATTATTCCTTATGCCGCTGTAGGCTGGAGCTGCTTCG |
| 58 | delparF | CGACCTGGTGAGCCTGGCCGAAGGCCAAAAGCCACTGCAAAACCGAAAAAATTCCGGGGATCCGTCGACC |
| 59 | delparR | AGACGCTAGCAAATTGCGAATCCTGCCCTAGTTCTAACCCCCCCATGTTTTGTAGGCTGGAGCTGCTTCG |
| 60 | deloriF | AACCGAAAAATTTCGTACGTACGAAAAAACATGGGGGGGTTAGAACTAGGATTCCGGGGATCCGTCGACC |
| 61 | deloriR | GGGGGACAGGTGCAATTTTAGCACAAGCGGCGGCAGACGCTAGCAAATTGTGTAGGCTGGAGCTGCTTCG |
| 62 | oriparF | GCCGAAGGCCAAAAGCCACTGCAAAACCGAAAAATTTCGTACGTACGAAAATTCCGGGGATCCGTCGACC |
| 63 | delkfrAF | ATGTATTGTATTAAAATACAATACATACAATACAGGGAGCCGAAGCCATGTGTAGGCTGGAGCTGCTTCG |
| 64 | delkfrAR | CACTTTATCTGTTTACGTCAATAGATAGGGGTTACTCTTTGGTGTCGGCTGCATGGGAATTAGCCATGG |
| 65 | delkfrCF | CCTGGCAGGTTTCGGGGCTATATGGGACGCTGACCGGGATTGAAACCATGTGTAGGCTGGAGCTGCTTCG |
| 66 | delkfrCR | AATGGCCGGGGTCGGTGACAGGGTAGCGGCTTCACCGCTCTAGATCGTCTTCATGGGAATTAGCCATGG |
| 67 | Kmpar1F | GGTGCAAAGACGCCGTGGAAGCGTGTGAGGTTGACTCGCGGCTTAGGTACATTCCGGGGATCCGTCGACC |
| 68 | Kmpar2R | TGTAGGCTGGAGCTGCTTCG |
| 69 | Kmpar3F | cgaacgagctccagcctacaCTTGCATGCCTGCAGGGTAC |
| 70 | Kmpar4R | CCCATGTGATCTTCGAGCCGCTGGACTTCATCGCCAAACTCGCTGCGTTGGGTACCCTGCCGGGGTTCTC |
Start and stop codons are marked in bold, the introduced restriction sites or overhangs are underlined, nucleotides not complementary to the template are shown in small letters, an additional Shine–Dalgarno sequence is underlined, and oligonucleotides used for mutagenesis [30] sequence corresponding to RA3 is in italics.