Figure 1.

RECK over expression is associated with delayed SiHa and SW756 derived tumor establishment, reduced tumor growth and increased survival in nude mice. (A). Differential tumor growth experiment in nude mice. Here, the experiment ended once most of the animals from the control group presented tumors with 500 mm³. (B–G). The effect of RECK over expression on cervical cancer derived cells tumor establishment and overall survival was assessed in vivo. SiHa (B,D,F) and SW756 (C,E,G) cells over expressing RECK were subcutaneously injected in nude mice (2 × 10⁶ cells/mouse). Tumors were identified as established once they were measurable with a manual caliper and the kinetics of tumor establishment for each of the cell lines tested are illustrated in B and C. D and E. Representative data from a tumor growth kinetics experiment. Tumor volume was measured weekly for at least 150 days. Overall survival was determined as each animal presented a tumor volume of 500 mm³ during the 150 days of experiment (F and G). * p < 0.05 by Mann–Whitney (1A), Log rank (1B, 1C, 1F and 1G) or two-way ANOVA (1D and 1E) test.