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. 2021 Apr 28;22(9):4660. doi: 10.3390/ijms22094660

Figure 6.

Figure 6

6-Gingerol regulates EGFR/Src/STAT3 and p53 signaling. (A) Molecular docking using AutoDock Vina software that shows the binding of 6-gingerol (PubChem ID: 442793) to the ATP-binding domain of EGFR (PDB ID: 2GS2). (B) Western blotting of MDA-MB-231 and MCF-7 cells pre-treated with recombinant EGF (25 ng/mL) for 1 h and then treated with 200 µM 6-gingerol for 48 h showing the expression patterns of phosphorylated EGFR and total EGFR proteins. Representative expression of proteins was determined by densitometry and normalized to GAPDH. Controls are set to 100. Data were confirmed after repeating the experiment three times. *** p < 0.001 (ANOVA test). # p < 0.001 vs. control. (C) Western blotting of MDA-MB-231 and MCF-7 cells following 48 h treatment with 100 and 200 µM of 6-gingerol showing expression levels for pEGFR, EGFR, pSrc, Src, pSTAT3, and STAT3. (D) Representative expression of proteins was determined by densitometry and normalized to GAPDH. Controls are set to 100. Data were confirmed after repeating the experiment three times. *** p < 0.001 (ANOVA test). # p < 0.001 vs. control. (E) Western blotting of MDA-MB-231 and MCF-7 cells pre-treated with STAT3 siRNA (30 pM) for 24 h and then treated with 200 µM 6-gingerol for 48 h showing the expression patterns of phosphorylated STAT3 and p53 proteins. (F) Representative expression of proteins was determined by densitometry and normalized to GAPDH. Controls are set to 100. Data were confirmed after repeating the experiment three times. *** p < 0.001 (ANOVA test). # p < 0.001 vs. control.