Skip to main content
. 2021 Apr 28;22(9):4660. doi: 10.3390/ijms22094660

Figure 7.

Figure 7

6-Gingerol induces p53-dependent apoptosis. (A) Western blotting of total proteins isolated from MDA-MB-231 and MCF-7 cells after 48 h treatment with 100 and 200 µM of 6-gingerol showed the expression of p53, pMDM2, and MDM2 proteins. (B) Representative expression of proteins was determined by densitometry and normalized to GAPDH. Controls are set to 100. Data were confirmed after repeating the experiment three times. *** p < 0.001 (ANOVA test). # p < 0.001 vs. control. (C) Western blotting of total proteins isolated from MDA-MB-231 and MCF-7 cells after 48 h treatment with 200 µM of 6-gingerol, p53 siRNA, and p53 siRNA + 6-gingerol showing the expression of p53, BAX, and BCL-2 proteins. (D) Representative expression of proteins was determined by densitometry and normalized to GAPDH. Controls are set to 100. Data were confirmed after repeating the experiment three times. * p < 0.05, ** p < 0.01, and *** p < 0.001 (ANOVA test). (E) Flow cytometry analysis using annexin V and PI staining in MDA-MB-231 and MCF-7 cells after treatment with 6-gingerol, p53 siRNA, and p53 siRNA + 6-gingerol for 48 h.