Figure 4.

Fluorescence detection by the Celena S Digital Imaging System of protein associates formed upon PARylation reaction catalysed by PARP1, PARP2, and their equimolar mixture. The proteins labelled with distinct fluorophores (AF-PARP2, Cy3-PARP1) were visualized in images acquired with appropriate filters (GFP for AF and RFP for Cy3); the overlapped signals for distinct fluorophores were visualized in the superimposed image (merge). Scale bar, 50 μM. Preparation of samples is detailed in Materials and methods.