Table 4.
DLS analysis of PARylated PARP2.
| Protein(s) 1 | RH 2, (nm) | |||
|---|---|---|---|---|
| Before Reaction Initiation | PARylated Protein Associate 3 | PARylated Protein after Addition of EDTA 3 | PARylated Protein after PARG Treatment 3 | |
| PARP2 | 5.0 ± 0.4 | 383 ± 45 | 5.6 ± 0.3 | 5.2 ± 0.3 |
| PARP2 + Polβ | 5.6 ± 0.3 | 370 ± 43 | 6.2 ± 0.5 | 5.8 ± 0.2 |
| PARP2 + APE1 | 4.9 ± 0.2 | 440 ± 51 | 5.2 ± 0.3 | n. d. 4 |
| PARP2 + XRCC1 | 7.6 ± 0.3 | 11.3 ± 1.6 | 6.7 ± 0.5 | 5.8 ± 0.4 |
| PARP2 + PARP1 | 7.8 ± 0.4 | 14.8 ± 1.4 | 9.9 ± 1.2 | n. d. 4 |
1 The concentration of proteins (individual and in the equimolar mixtures) was 6 μM. 2 Experimentally determined average RH value of species in single (major) peak of volume-weighted size distribution. Values are the mean (± SD) of three independent experiments. 3 Poly(ADP-ribose) synthesis was performed in the presence of Mg2+ for 15-min incubation; further treatment with EDTA and PARG was performed as detailed in Materials and methods. 4 Not determined.