Table 4.
Location | Conserved Motif | Functional Role | Human ABCG2 | Candida albicans Cdr1 | |
---|---|---|---|---|---|
First Half | Second Half | ||||
NBD | Walker A | ATP hydrolysis (phosphate binding) * | G79–S88 | G187–T195 | G895–T903 |
Q-loop | TMD–NBD communication | Q126 | E238 ** | Q942 | |
Hot spot | Triple helical bundle | L134–A149 | L246–P261 | S950–S965 | |
Signature | NBD dimerization and phosphate binding | V186–R193 | V303–R310 | V996–R1008 | |
Pro loop | NBD dimerization | P204 | ? | P1019 | |
Walker B | ATP hydrolysis | I206–E211 | I323–N238 | L1021–E1027 | |
D-loop | NBD dimerization | L216–D217 | L333–D334 | L1032–D1033 | |
H-loop | ATP hydrolysis | H243 | Y361 ** | H1059 | |
Elbow helix | Conserved R | Salt bridge, THB | R383 | R503 | R1185 |
ECL1 | Conserved R | Salt bridge | R426 | R456 | ? |
TMH2 | Conserved F | Clamping | F439 | F559 | F1239 |
ICL1 | Conserved E (1) | Salt bridge and intracellular gating | E451 | E570 | D1255 |
Conserved E (2) | Salt bridge | E458 | E576 | E1261 | |
Conserved Y | Salt bridge, THB | Y464 | Y584 | Y1257 | |
TMH3 | Conserved D/E | Intracellular gating | D477 | E597 | E1280 |
Valve | Conserved hydrophobic | Valve | G553–L555 | G672–V674 | G1362–L1364 |
Re-entry helix | Conserved P | Kinked helix | P574 | P692 | P1382 |
Conserved E | Salt bridge | E585 | E704 | ? | |
ECL3 | Conserved C (1) | Intra/intermolecular disulfide bond | C592 | C712 | C1418 |
conserved C (2) | Intra/intermolecular disulfide bond | C603 | ? | C1441 | |
conserved C (3) | Intra/intermolecular disulfide bond | C608 | C732 | C1444 |