Table 1.

Kinetic constants for the site-directed mutagenesis HepI.

HepI	kcat (s−1)	Fold Change	KM (M)	Fold Change	kcat/kM
Wild type	0.59 ± 0.09	-	8.5 ± 3.6	-	6.8 × 104
P216G	0.21 ± 0.02	2.8 ↓	1.6 ± 0.6	5.6 ↓	1.3 × 105
P240G	0.19 ± 0.01	3.1 ↓	0.9 ± 0.3	10 ↓	2.0 × 105
G280P	0.21 ± 0.02	2.8 ↓	1.5 ± 0.5	6.0 ↓	1.3 × 105
G288P	0.48 ± 0.07	1.2 ↓	10.2 ± 0.4	1.1 ↑	4.7 × 104