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. 2021 Apr 28;22(9):4623. doi: 10.3390/ijms22094623

Figure 5.

Figure 5

PD173074 inhibition affects cellular amount of FGFR2 in RS4;11 cells. RS4;11 cells were serum-starved for 12 h and preincubated for 1 h (A) with DMSO alone, (B) with DMSO and then stimulated with 0.1 ng/mL FGF2, or (C) with 2 µM of PD173074 and then stimulated with 0.1 ng/mL FGF2. Thirty micrograms of cytosolic/membrane proteins (C/M) and 50 µg of nuclear proteins (N) were analyzed by western blot with anti-FGFR2 antibody; αTubulin and Lamin B were used as loading control of cytosolic and nuclear proteins, respectively (upper panels); whole cellular extract (WCE). Immunofluorescence was performed with an anti-rabbit CY2 antibody to evaluate the localization of FGFR2, and DAPI, for nuclear staining (bottom panels). Scale bars: 10 μm.