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. 2021 Apr 29;22(9):4738. doi: 10.3390/ijms22094738

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© 2021 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Identification of protein-interacting domains of AtC3H59 and Desi1. (a) Schematic maps of full-length ORF of AtC3H59 and truncated fragments of AtC3H59 for yeast two-hybrid with full-length Desi1. (b) Quantitative β-galactosidase ONPG assay. The interaction was quantified by measuring the β-galactosidase activity in yeast extract. (c) Yeast growth assay. Yeast transformants were grown on SM-Trp/-Leu/-Ura. (d) Schematic maps of full-length ORF of Desi1 and truncated fragments of Desi1 for yeast two-hybrid with C-terminal region of AtC3H59. (e) Quantitative β-galactosidase ONPG assay. The interaction was quantified by measuring the β-galactosidase activity in yeast extract. (f) Yeast growth assay. Yeast transformants were grown on SM-Trp/-Leu/-Ura. In (b,e), data shown are the means ± S.D. (n = 3). * t-test p < 0.05. In (b,c,e,f), pBD-GAL4 vector and pGADT7 were used as a bait and a prey for a negative control, respectively. NC, negative control; F, full-length ORF; N, N-terminal region; M, middle region; C, C-terminal region.