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Main differences between 2D and 3D cultures.
| Advantages and Disadvantages | 2D Culture | 3D Culture |
|---|---|---|
| Chemical gradient formation | − | + |
| Physiological architecture | − | + |
| 3D cell migration/interaction | − | + |
| Drug resistance | − | + |
| In vivo-like gene expression | −/+ | + |
| Protocol standardization | + | − |
| Reproducibility | + | +/− |
| Comparison in scientific literature | + | +/− |
