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. 2021 Apr 30;22(9):4776. doi: 10.3390/ijms22094776

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© 2021 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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(A) LC–MS/MS based bottom-up proteomic workflow. Platelets isolated from blood are subjected to lysis, enzymatic digestion, separation of peptides on a reversed-phase LC column, ionized (ESI), and mass analyzed (MS). (B) Modes of MS data acquisition. Three main strategies for data acquisition: data-dependent acquisition (DDA), data-independent acquisition (DIA), and targeted acquisition by multiple or parallel reaction monitoring (MRM or PRM). The DDA and DIA are aimed to analyze global proteomic changes, whereas the targeted methods focused on monitoring preselected target proteins with high precision and accuracy.