FIG 2.
Shotgun proteomic analysis of M. smegmatis wild-type and terminal oxidase mutants at mid-log phase grown in air in the presence and absence of 20% CO. (A to D) Volcano plots showing differential abundance of proteins in (A) wild-type, (B) ΔqcrCAB, (C) ΔcydAB and (D) ΔdosR strains harvested at mid-exponential phase (OD600, ∼0.3) grown in air with and without 20% CO. Each protein is represented by a single point, with dos regulon proteins represented by black dots and proteins of interest highlighted as per the legend. Dotted lines represent significance and fold change thresholds. (E and F) Venn diagrams showing proteins identified by the shotgun proteomics in multiple data sets with higher (E) and lower (F) abundances in wild-type, ΔqcrCAB, ΔcydAB, and ΔdosR mutant strains grown in air plus 20% CO.