Table 3.

qPCR conditions for AT₁ and AT₂ receptors’ expression analysis.

Genes of Interest
AT1 receptor	Forward	5′-TCTGGATAAATCACACAACCCTC-3′
	Reverse	5′-GAGTTGGTCTCAGACACTATTCG-3′
AT2 receptor	Forward	5′-CTGGCAAGCATCTTATGTAGTTC-3′
	Reverse	5′-ACAAGCATTCACACCTAAGTATTC-3′
Housekeeping Genes
Papbn-1	Forward	5′-TATGGTGCGACAGCAGAAGA-3′
	Reverse	5′-TATGCAAACCCTTTGGGATG-3′
Hprt-1	Forward	5′-CCCAGCGTCGTGATTAGTGATG-3′
	Reverse	5′-TTCAGTCCTGTCCATAATCAGTCC-3′
Hmbs	Forward	5′-TCTAGATGGCTCAGATAGCATGCA-3′
	Reverse	5′-TGGACCATCTTCTTGCTGAACA-3′