Figure 1.

Effects of paeonolide (PALI) isolated from the roots of P. suffruticosa on cytotoxicity and proliferation in pre-osteoblasts. (A) ¹H and ¹³C NMR spectra of PALI from the roots of P. suffruticosa. (B) HPLC chromatogram of PALI. (C) Chemical structure of PALI. (D) Pre-osteoblasts were treated with PALI at concentrations ranging from 0.1 to 100 μM for 24 h, and cell viability (%) was measured using an MTT assay. Data are representative of the results of three independent experiments and values are expressed as mean ± SEM.