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. 2021 May 6;22(9):4924. doi: 10.3390/ijms22094924

Figure 4.

Figure 4

Effects of paeonolide (PALI) on BMP2, Wnt3, and MAPKs signaling during osteoblast differentiation. (AC) After pre-osteoblasts were treated with PALI or BMP2 (100 ng/mL) during osteogenic supplement medium (OS)-induced osteoblast differentiation for 24 h, equal amounts of lysates were analyzed using Western blot analysis and detected with antibodies against BMP2, phospho-Smad1/5/8 (p-Smad1/5/8) (A); Wnt3a, phospho-GSK3β (p-GSK3β), and β-catenin (B); and phospho-ERK (p-ERK), ERK, phospho-JNK (p-JNK), JNK, phospho-p38 (p-p38), and p38 (C). β-actin was used as a loading control. BMP2 was used as a positive control. Data are representative of the results of three independent experiments. Bar graphs are represented as relative percentages of the control. Data are representative of the results of three independent experiments and values are expressed as mean ± SEM. *, p < 0.05 indicates a statistically significant difference compared with the control; #, statistically significant difference compared with OS (p < 0.05); $, statistically significant difference compared with the OS + PALI (p < 0.05).