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. 2021 Apr 27;22(9):4604. doi: 10.3390/ijms22094604

Figure 2.

Figure 2

High glucose effects on cell proliferation and viability in human adipose mesenchymal stem cells cultured in basal medium (ASC) or in pericyte medium (pmASC), and in human retinal pericytes (HRPC). In some samples, glucose was added to the culture medium (High Glucose, 25 mM, HG); data were gathered after 24 h, 48 h and 72 h from glucose addition and compared to corresponding samples kept in normal glucose (NG) condition. Proliferation rate was assessed by crystal violet assays in ASC, pmASC (A), and HRPC cultures (B) in NG or in HG. Cell viability was evaluated by MTT assays in ASC, pmASC (C), and HRPC cultures (D) in NG or in HG. Absorbance values were determined at 570 nm for both assays. Values are expressed as mean ± SEM of three independent experiments. In A and C, values are referred to ASC NG population, at each corresponding time point. In B and D, values are referred to HRPC NG population, at each corresponding time point. * p < 0.05 pmASC vs. ASC; # p < 0.05 HG vs. NG. Two-way ANOVA, followed by Sidak’s test.