Figure 6.

High glucose effects on tube-like structure formation by human retinal endothelial cells (HREC), or co-cultured with ASCs (HREC + ASC) or with pmASCs (HREC + pmASC). In some samples, glucose was added to the culture medium (High Glucose, 25 mM, HG) and compared to samples kept in normal glucose (NG) condition. (A), Representative microphotographs showing three-dimensional cultures in Matrigel of each sample. Pictures were taken at 6 h after seeding. Scale bar: 250 µm (Magnification: 4×). Histograms at the bottom show the “total master segment length” (B), the “total isolated branch length” (C) and “branching points” (D). Quantitative analysis was carried out using Image J software. Values are expressed as a mean ± SEM of three independent experiments. * p < 0.05 pmASC vs. ASC; # p< 0.05 HG vs. NG; § p< 0.05 vs. HREC HG. Two-way ANOVA, followed by Sidak’s test.