Figure 12.

(A) Schematic illustration of molecular phenotype profiling of CD63-positive exosomes using CD63 antibody-functionalized magnetic beads and three classes of SERS-encoded nanoparticles separately conjugated with antibodies targeting Glypican-1, EpCAM, and CD44V6 surface biomarkers. SERS codes: 5,5′-dithiobis(2-nitrobenzoic acid) (DTNB); 4-mercaptobenzoic acid (MBA), and 2,3,5,6-Tetrafluoro-4-mercaptobenzonic acid (TFMBA). (B) SERS spectra for the simultaneous detection of three biomarkers on Panc-1-derived exosomes in PBS. Peaks at ca. 1077, 1340, and 1380 cm⁻¹ are correlated with the presence of EpCAM, Glypican-1, and CD44V6, respectively. (C) Phenotypic signature of Panc-1-, C3-, and SW480-derived exosomes in PBS and plasma (n = 3). Adapted with permission from [97]. Copyright 2020, American Chemical Society.