Figure 2.

(a) Specific activity of recombinant CPT II wild type and variants (enzyme concentration 1 mg/mL during incubation time) at 30 °C measured spectroscopically using enzyme assay. The reaction was started by adding 15 nM enzyme. (b) Half-lives of wild type and variants (1 mg/mL) at different temperatures. Error bars are standard deviation (SD) derived from three independent experiments. The statistical differences of the half-lives between WT and the variants and between the different temperatures are given as p-values in Tables S1 and S2.