Figure 3.

The effects of the GPR4 antagonist NE52-QQ57 on pro-apoptotic Bax and anti-apoptotic Bcl-2 protein expressions in MPTP-treated mice. MPTP was administered (30 mg/kg/day) for 5 days. Mice were sacrificed 3 days after the final MPTP injection, and the substantia nigra and striatum tissues were collected. (a) Bax and Bcl-2 protein expression in the SNpc region of the mouse brain (n = 3) and densitometric analysis. (b) Bax and Bcl-2 protein expression in the striatum region of the mouse brain (n = 3) and densitometric analysis. β-Actin was utilized as an internal control. Data are presented as the mean ± SEM. One-way ANOVA, followed by Tukey’s multiple comparison test, was used. # p < 0.05 when the MPTP-treated group was compared with the vehicle-only group; * p < 0.05 when other treated groups were compared with the MPTP-treated group.