Figure 10.

(a) The viability of murine fibroblasts L-929 after 24 h incubation with WPI modified HAp A and HAp B biocomposites, evaluated using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) reduction assay according to ISO-10993-5:2009. The cells incubated without composites served as a positive control of the viability (100%). The data are presented as mean ± SD for the three separate experiments. The green line indicates the minimum level (70%) of the cells’ metabolic activity required to recognize the biomaterial as non-cytotoxic at the in vitro level; (b) NF-κB induction in THP1-Blue™ monocytes incubated for 24 h with WPI modified HAp A, and HAp B biocomposites. The cells incubated without composites served as a negative control of the monocyte’s activation (NC), and monocytes stimulated with S. cerevisiae β-glucan served as the positive control (PC). The data are presented as the mean ± SD for the four separate experiments. The green line indicates the physiological level (0.114 ± 0.013) of the non-stimulated monocytes. * p values (<0.05) calculate in comparison to the untreated cell cultures.