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. 2021 Apr 28;22(9):4680. doi: 10.3390/ijms22094680

Figure 2.

Figure 2

Label-free LC–ESI–MS/MS experimental design and main results. Protein extracts from 15 male and 15 female mice fed for 14 weeks with a standard diet (10 kcal% fat content; CTR) and two high-fat diets (HFD) regimens (45 kcal% and 60 kcal% fat content diets; 45% HFD and 60% HFD, respectively). For both genders, 5 samples for each condition were collected and analyzed in duplicate using label-free LC–ESI–MS/MS protocol. Three different comparisons were performed: 45% HFD vs. CTR, 60% HFD vs. CTR and 45% HFD vs. 60% HFD. The ANOVA test (n = 10, p-value > 0.05) showed 160 and 46 proteins differentially regulated in males and females, respectively. Of them, 38 proteins were in common between males and females. Specifically, comparing 45% HFD vs. CTR, 117 and 33 differentially abundant proteins were found, in males and females, respectively (Tukey’s multiple comparison test, p-value < 0.05). In contrast, the same analysis highlighted 131 and 41 differentially abundant proteins in 60% HFD vs. CTR males and females, and 119 and 9 differentially abundant proteins in 45% HFD vs. 60% HFD males and females, as detailed in Supplementary Tables S1 and S2.