Figure 2.

TET2 KO does not alter the DNA methylation and hydroxymethylation level at the promoter region of PD-L1 gene. (A) Snapshot of TET2 ChIP-seq data (GSE120756) at PD-L1 (CD274) promoter in WT and TET2 KO MCF7 cells. (B) ChIP-qPCR validation of TET2 occupancy at PD-L1 promoter in WT and TET2 KO MCF7 cells. (C) MeDIP-qPCR analysis of 5mC enrichment at PD-L1 promoter in WT and TET2 KO MCF7 cells. (D) hMeDIP-qPCR analysis of 5hmC enrichment at PD-L1 promoter in WT and TET2 KO MCF7 cells. (E) RT-qPCR analysis of the relative mRNA expression levels of PD-L1 in WT and TET2 KO MCF7 cells with or without 10 μM 5-Aza treatment. (*, p < 0.05; ***, p < 0.001; ns, not significant.)