Figure 1.

Graphic representation of the experimental protocol. Anesthetized, instrumented piglets were assigned to either the vehicle-treated (VEH), SZR72-treated (SZR72), or the hypothermia-treated (HT) groups. After obtaining baseline (BL) blood gases, serum samples, and visual evoked potential (VEP) recordings, the animals were exposed to asphyxia induced by ventilation with a hypoxic/hypercapnic gas mixture for 20 min. SZR72 or vehicle administration started 5 min upon completion of asphyxia, whereas in the HT group cooling started simultaneously with reoxygenation, rectal temperature (T_rect) reached 33.5 °C in 40–50 min. Blood gases, serum samples, and VEP recordings were obtained at the indicated time points. At the end of the observation period, the brains were processed for neuropathology examination.