Table 1.
Main UHPLC–MS/MS parameters of MRM transitions of each analyte.
| Compound | Retention Time (min) | Retention Time Window (min) | Precursor Ion (m/z) | Type of Product Ion | Product Ion (m/z) | Collision Energy (eV) | RF Lens (V) |
|---|---|---|---|---|---|---|---|
| Kynurenine | 1.45 | 1.0 | 209.2 | quantifier | 192.1 | 10 | 52 |
| Kynurenine | 1.45 | 1.0 | 209.2 | qualifier | 146.0 | 19 | 52 |
| SZR72 | 2.43 | 0.4 | 260.1 | quantifier | 215.0 | 18 | 75 |
| SZR72 | 2.43 | 0.4 | 260.1 | qualifier | 144.0 | 32 | 75 |
| SZR73 (IS) | 2.57 | 0.4 | 274.2 | quantifier | 144.0 | 37 | 65 |
| SZR73 (IS) | 2.57 | 0.4 | 274.2 | qualifier | 229.0 | 17 | 65 |
| KYNA | 2.84 | 0.45 | 190.1 | quantifier | 144.0 | 19 | 55 |
| KYNA | 2.84 | 0.45 | 190.1 | qualifier | 172.0 | 13 | 55 |