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. 2021 Apr 27;26(9):2551. doi: 10.3390/molecules26092551

Figure 3.

Figure 3

Efficacy of acute VDA activity revealed using dynamic BLI in orthotopic breast tumors. Left: BLI signal intensity images overlaid as heat maps on gray scale photographs of mice at about 10 min after administration of luciferin at selected time points following administration of (a) saline or (b) CA4P (120 mg/kg IP) to MDA-MB-231-luc xenograft tumor bearing nude mice. See also Video S1; (c) CA4P to syngeneic 4T1-luc tumor in BALB/C mouse. All intensity maps have same heat scale. Center: corresponding BLI intensity curves for the respective individual mice at left showing differential variation over a period of 35 min following administration of luciferin at baseline (red), 2 h post (orange), 4 h (green) and 24 h (blue), 48 h (purple). (d) Tumor sections from four tumors showing vascular extent based on CD31 stain (green) and perfusion marker Hoechst 33342 (blue) at different times following treatment with CA4P. Severely diminished perfusion was seen at 4 h, while controls tumors showed highly consistent extensive perfusion as seen in right hand column. Scale bar: 50 µm.