Figure 3.

Cleavage of the [³²P]-RNA target fragment of the EGFR gene hybridized with triped 1 and with ASO-22 monitored electrophoretically (PAGE) under in vitro conditions in the presence of recombinant RNase H (A); silencing activity of ASO-22 and 1/2 towards the endogenous mRNA of EGFR protein in A431 cancer cells analyzed by immunoblot imaging ((B,D), respectively) and quantified in the plot (C) * p ≤ 0.05 and (E) **** p ≤ 0.0001 (ANOVA and post-hoc Tukey HSD test), respectively. A431 cells transfected with ASO-C are the control and M is the ³²P-isotope labeled mixture of oligoribonucleotides (2–22 mers).