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. 2021 Apr 28;22(9):4668. doi: 10.3390/ijms22094668

Figure 1.

Figure 1

The upregulated TAP1 in response to Toll-like receptors (TLRs) agonists and viral infections. (A–E) Raw 264.7 cells were stimulated with LPS (1 μg/mL), polyI:C (25 μg/mL), R848 (100 nM), IFN-α (2000 U/mL), and HSV-1 (MOI = 0.25), respectively, and then the expression of TAP1 gene was measured by RT-qPCR at different timepoints. The expression level of mRNA was normalized to the expression of β-actin, and the data from at least triplicates were shown as the mean ± SD. * p < 0.05, ** p < 0.01, *** p < 0.001. (F) Wild type bone marrow-derived macrophage (WT-J2-BMM) cells or interferon α receptor-deficient (IFNAR−/−)-J2-BMM cells were stimulated with LPS (1 μg/mL), polyI:C (25 μg/mL), R848 (100 nM), R848 (100 nM), IFN-α (2000 U/mL), and HSV-1(MOI = 0.25) for 24 h, respectively, and then the expression of TAP1 protein was measured by Western blot analysis. GAPDH was used as intern control. The relative ratios of TAP1 and GAPDH were marked at the bottom of the pictures.