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. 2021 Apr 28;22(9):4668. doi: 10.3390/ijms22094668

Figure 2.

Figure 2

Broadly antiviral activities of TAP1. (A) HEK293T cells were transfected with TAP1-expressing plasmid in different concentrations (2.5 μg/mL, 1.25 μg/mL, 0.6 μg/mL, and 0.3 μg/mL) for 24 h, followed by HSV-1 infection (MOI = 1) for 8 h. The expression level of TAP1 and HSV-1 UL27 was quantified by RT-qPCR. (B) HEK293T cells were transfected with TAP1-expressing plasmid in different concentrations (2.5 μg/mL, 1.25 μg/mL, and 0.6 μg/mL) for 24 h, followed by Ad5 infection (MOI = 1) for 8 h. The expression level of TAP1 and Ad5 was quantified by RT-qPCR. (C) HEK293T cells were transfected with TAP1-expressing plasmid in different concentrations (0.5 μg/mL, 0.25 μg/mL, and 0.125 μg/mL) for 24 h, followed by VSV infection (MOI = 1) for 8 h. The expression level of TAP1 and VSV was quantified by RT-qPCR. (D) HEK293T cells were transfected with TAP1-expressing plasmid in different concentrations (2 μg/mL, 1 μg/mL, and 0.25 μg/mL) for 24 h, followed by PR8 infection (MOI = 1) for 8 h. The expression level of TAP1 and PR8 was quantified by RT-qPCR (E) HEK293T cells were transfected with TAP1-expressing plasmid in different concentrations (5 μg/mL, 2.5 μg/mL, and 1.25 μg/mL) for 24 h, followed by ZIKV infection (MOI = 1) for 8 h. The expression level of TAP1 and ZIKV was quantified by RT-qPCR. (F) HEK293T cells were transfected with TAP1-expressing plasmid in different concentrations (5 μg/mL, 1.25 μg/mL, and 0.3 μg/mL) for 24 h, followed by DENV4 infection (MOI = 1) for 8 h. The expression level of TAP1 and DENV4 was quantified by RT-qPCR. The expression level of mRNA was normalized to the expression of β-actin, and data from at least triplicates were shown as the mean ± SD. * p < 0.05, ** p < 0.01, and *** p < 0.001. (G–J) HEK293T cells were transfected with TAP1-expressing plasmid in indicated concentrations for 24 h, followed by Ad5, VSV, ZIKV, and DENV4 infection (MOI = 1) for 8 h, respectively. The expression level of TAP1 and GAPDH was quantified by Western blot, and the relative ratios of TAP1 and GAPDH were labeled at the bottom of the pictures. GAPDH was used as the internal control.