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. 2021 May 5;22(9):4902. doi: 10.3390/ijms22094902

Figure 4.

Figure 4

Specific detection of TGFβ3 in human cumulus cells shows no significant difference with age. (A) Three representative images of the varying sizes of cumulus cell masses from smaller to larger are shown from participant 42 (39 y). All cumulus cell images were taken upon receipt to the lab. (B) We observed a specific immunoreactive band for TGFβ3 at 50 kDa representing the pro-form of the TGFβ3 molecule. MCF7 is the positive control. Two intact cumulus cell masses from participant 31 are shown in lanes 2 and 3. The arrow indicates TGFβ3 (50 kDa). (C) Representative blot probed with antibodies recognizing TGFβ3 and reprobed for GAPDH (36 kDa). (D) Ratio of TGFβ3 to GAPDH in young and old cumulus cells for all replicates. Young and old groups N.S. (E) Fold-change of TGFβ3 in old cumulus cells compared to young cumulus cells for all replicates. More detailed information about each participant can be found in Supplementary Table S1. Scale bar = 100 µm.