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. 2021 Apr 28;10(9):1901. doi: 10.3390/jcm10091901

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© 2021 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Immuno-gold EM of cryo-sections from formaldehyde-fixed duodenal biopsy and enteroid samples from MYO5B-PFIC patient #1. Corresponding mislocalization patterns of apical markers to aberrant subapical RE are evident in biopsy #1 (a,c) and enteroid (b,d); all scale bars = 500 nm. (a) Rab11 in biopsy visualized by 1.4nm Nanogold plus silver enhancement. (b) Rab11a in enteroid visualized by 5nm colloidal gold (arrows). (c) NHE3 in biopsy (enhanced Nanogold), (d) NHE3 in enteroid (enhanced Nanogold).