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. 2021 Apr 28;10(9):1901. doi: 10.3390/jcm10091901

Figure 10.

Figure 10

Immuno-gold EM of cryo-sections and 3D reconstruction of subapical vesicle clusters in hepatocytes of biopsy from MYO5B-PFIC patient #1 (a,c–f) plus labeling control (b). (a) BSEP immuno-gold label (arrows) appears weak at the apical plasma membrane but abnormally enriched in the cytoplasm and/or at vesicles around the bile canaliculus; scale bar = 500 nm. (b) Bona fide normal BSEP distribution (arrows) concentrated at the plasma membrane of control hepatocytes; scale bar = 500 nm. (c) Very moderate, local mislocalization of MRP2 (arrows) in biopsy of patient #1; scale bar = 500 nm. (d–f) Electron tomographic reconstruction of 3D-architecture of subapical vesicles (plus rER cisternae) in hepatocytes of aldehyde-fixed, HPF/FS sample from patient #1. 2D slice (d) from an electron tomographic reconstruction with contour lining (e) of vesicles (red), rER (blue), plasma membrane (green) and the resulting 3D model (f); scale bars = 600 nm.