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. 2021 Apr 30;22(9):4788. doi: 10.3390/ijms22094788

Figure 2.

Figure 2

AP collagen peptides and their major component GPH prevent cortisol-dependent downregulation of collagen type I. HDFs were treated with cortisol (100 nM) in the presence of various concentrations of AP collagen peptides (APCP, 0, 0.1 and 1 μg/mL) or various concentrations of GPH (0, 0.01 and 0.03 μg/mL) and cultured for 24 h. (A,C) Total RNA was extracted from cells and relative col1a1 mRNA levels were measured by qRT-PCR. (B,D) Culture media were harvested and the level of procollagen type I was detected using a specific ELISA kit. All data represent the mean ± SD of three independent experiments. Significant differences: # p < 0.05, ## p < 0.01, ### p < 0.005 compared with the control; * p < 0.05, ** p < 0.01, *** p < 0.005 compared with cortisol-treated control.