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. 2021 Apr 30;22(9):4788. doi: 10.3390/ijms22094788

Figure 5.

Figure 5

AP collagen peptides alleviate cortisol-dependent inhibition of procollagen type I in replicative senescent HDFs. (A) Young HDFs at passage 5 (P5) and replicative senescent HDFs at passage 25 (P25) were stained for the presence of senescence-associated beta-galactosidase (SA-β-gal) activity (blue). Scale bar = 200 μm. (B) The percentage of senescent cells was determined for SA-β-gal. Senescent HDFs were treated with cortisol (100 nM) in the presence of APCP (1 μg/mL) or GR inhibitor (1 μM) and cultured for 24 h. (C) Culture media from cells cultured for 24 h were harvested and the levels of procollagen type I were determined via a specific ELISA kit. (D) Total RNA was extracted from young HDFs and senescent HDFs, and relative GR mRNA was measured via qRT-PCR. All data represent the mean ± SD of three independent experiments. Significant differences: ## p < 0.01, ### p < 0.005 compared with control; * p < 0.05, ** p < 0.01 compared with cortisol-treated control.