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. 2021 May 7;13(9):2250. doi: 10.3390/cancers13092250

Figure 3.

Figure 3

IGF2 limits the response of HNCHPV+ cells to isiPI3K. (A) UM-SCC47 and UT-SCC60A sensitive cells were transfected with either si-Control (siCT) or si-RNA targeting IGF2 (siIGF2) in the presence of DMSO, GDC0032, or BYL719. Cell viability was determined 4 days post-treatment using crystal violet staining. siRNA results of 2 independent experiments are presented as means ± SEM. Biological replicates from separate experiments are shown as grey dots. (B) UM-SCC47 and UT-SCC60A sensitive cells were cultured with DMSO, GDC0032, or BYL719, and AEW541, in the presence or absence of rIGF2. Cell viability was measured 4 days post-treatment using crystal violet staining. The proliferation experiment was repeated 3 times, and the results of a representative experiment are presented as means ± SEM. Biological replicates from one experiment are shown as grey dots. (C) UM- SCC47 and UT-SCC60A sensitive cells were cultured with DMSO, GDC0032 (500 nM), or BYL719 (2 μM), with or without rIGF2. Three days post-treatment, cell cycle analysis was performed. The fraction of cells in the S-phase was determined. Cell cycle results of 2–3 separate experiments are presented as means ± SEM. Biological replicates from separate experiments are shown as grey dots. Statistical significance was calculated by one-way ANOVA, * p < 0.05; ** p < 0.01; *** p < 0.001; **** p < 0.0001.