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. 2021 Apr 28;22(9):4648. doi: 10.3390/ijms22094648

Figure 2.

Figure 2

Additive effects of EP2 agonists to ripasudil (Rip) on the mRNA expression of ECMs of 2D cultured 3T3-L1 cells. The 2D cultures of 3T3-L1 cells were prepared under several sets of conditions: preadipocytes of 3T3-L1 cells (DIF-) or adipogenic differentiation (DIF+) with or without a combination of 10 μM ripasudil (Rip) and the EP2 agonist, 100 nM omidenepag (OMD) or 100 nM butaprost (Buta). These specimens were subjected to a qPCR analysis to estimate the expression of the mRNA of the major ECMs (Col1: collagen 1; Col4: collagen 4; Col6: collagen 6; Fn: fibronectin). All experiments were performed in triplicate using fresh preparations, and each experiment consisted of 5 samples. Data are presented as the arithmetic mean ± the standard error of the mean (SEM). * p < 0.05, ** p < 0.01, *** p < 0.005, **** p < 0.001 (ANOVA followed by a Tukey’s multiple comparison test).