Figure 3.

Additive effects of EP2 agonists to ripasudil (Rip) on the mean sizes of the 3T3-L1 3D organoids during adipogenesis. The 3D organoids of 3T3-L1 cells were prepared under several sets of conditions: preadipocytes of 3T3-L1 cells (DIF-) or adipogenic differentiation (DIF+) with or without a combination of 10 μM ripasudil (Rip) and the EP2 agonist, 100 nM omidenepag (OMD) or 100 nM butaprost (Buta). Their mean area sizes (μm²) were measured and plotted during a 7-day culture period (left panel) and those at Day 7 were compared among the experimental groups (right panel). All experiments were performed in triplicate using fresh preparations, each consisting of 16 organoids. Data are presented as the arithmetic mean ± the standard error of the mean (SEM). **** p < 0.001 (ANOVA followed by a Tukey’s multiple comparison test).