Figure 6.

Additive effects of EP2 agonists to ripasudil (Rip) on the mRNA expression of ECMs in 3T3-L1 3D organoids. The 3D organoids of 3T3-L1 cells were prepared under several sets of conditions: preadipocytes of 3T3-L1 cells (DIF-) or adipogenic differentiation (DIF+) with or without the combination of 10 μM ripasudil (Rip) and the EP2 agonist, 100 nM omidenepag (OMD) or 100 nM butaprost (Buta). The specimens collected at Day 7 were subjected to a qPCR analysis to estimate the expression of mRNA for the ECMs (Col1: collagen 1; Col4: collagen 4; Col6: collagen 6; Fn: fibronectin). All experiments were performed in duplicate using fresh preparations, each of which consisted of 16 organoids. Data are presented as the arithmetic mean ± the standard error of the mean (SEM). * p < 0.05, ** p < 0.01, **** p < 0.001 (ANOVA followed by a Tukey’s multiple comparison test).